Efficacy of selected purification techniques for bromelain
ثبت نشده
چکیده
Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase extraction, cation exchange chromatography and ammonium sulphate precipitation. Of the four techniques, cation exchange chromatography had shown the best bromelain purification technique with purification fold of 10.0 followed by reverse micellar system containing CTAB/ isooctane/ hexanol/ butanol, ATPE containing PEG polymer, ammonium sulphate precipitation and ATPE containing PEO-PPO-PEO with purification fold of 5.2, 4.0, 2.81 and 1.25, respectively.
منابع مشابه
Bromelain: Methods of Extraction, Purification and Therapeutic Applications
Bromelain is a concoction of sulfhydryl proteolytic enzymes. Depending upon the site of extraction it can be regarded as either stem bromelain (SBM) (EC 3.4.22.32) or fruit bromelain (FBM) (EC 3.4.22.33). Bromelain remain enzymatic active over a broad spectrumand endure a range of pH (5.5 to 8.0) and temperature (35.5 to 71 oC). It is one of the extensively investigated proteolytic enzyme owing...
متن کاملPartial purification of crude stem bromelain improves it sensitivity as a protease inhibitive assay for heavy metals
The protease inhibitive assay for heavy metals is a novel and robust detection method suitable for preliminary screening method for detecting heavy metals in agriculture and environment samples. Stem bromelain has been previously showed to be sensitive towards mercury and copper. However, commercial preparation is in crude form and contains possible interfering entities. Using ion exchange and ...
متن کاملIsolation and Purification of Bromelain from Waste Peel of Pineapple for Therapeutic Application
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...
متن کاملNeural Modeling of Bromelain Extraction by Reversed Micelles
A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the expe...
متن کاملCharacterization of Bromelain from Ananas Comosus Agroindustrial Residues Purified by Ethanol Factional Precipitation
Brazil produces over than 70,000 ha of pineapple, being the world leader in pineapple production, and Minas Gerais State alone produces over than 600 kt/y. The pineapple is the marketable plant part, which represents only 23 % of the plant itself, and the remaining plant, stem, leaves, bark and crown, being considered an agroindustrial residue. This residue contains great amount of bromelain, a...
متن کامل